Marla Glen grew up on the South Side of Chicago, the daughter of Dell Glen (American-Jamaican) and Cortez Glen (Mexican-American). Glen's father was a blues guitarist, her grandmother a gospel singer, and her mother was friends with the blues guitarist B.B. King. Her mother Dell graduated from the University of Chicago and went on to become a successful banker who worked for Merrill Lynch. Glen's father, an American patriot who joined the military at a young age, became an engineer based in Frankfurt, Germany. Like many other children in Chicago's historic Bronzeville neighborhood (also known as the Black Metropolis) who received gifts and inspiration from folks like Muddy Waters and B.B. King, Marla was given a toy harmonica as a child, and her musical talent became immediately apparent. She was only 11 years old when she wrote her first song, "Repertoire", a title that has won three gold records and one platinum, and appears on her 1996 release, Love and Respect.
Since 1998, she has lived in Heilbronn in Germany. On July 2, 2004, she entered a civil union with Sabrina Conley at the local Rathaus. In the meantime, they got divorced.